The Similar and Distinct Regulation of Nitric Oxide Synthase Isoform Expression in Mouse and Human Fallopian Tubes
Presentation Number: SUN-0045
Date of Presentation: June 22nd, 2014
State Key Lab of Medical Neurobiology, Shanghai, China
Nitric oxide (NO) is highly unstable, with a half-life of seconds in buffer solutions. It regulates the contractility and relaxation of Fallopian tube, and is involved in gametes maturation, fertilization, early embryonic development and embryo transfer to the uterus[1, 2]. NO is synthesized by the action of NO-synthase (NOS) with three isoforms of NOS being identified: neuro nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS).In the present study, we probed the expression and regulation of NOS in the mouse and human fallopian tube during the mouse estrous cycle and human menstrual cycle, together with NOS expression in the human tube after ectopic pregnancies as well as after lipopolysacharide (LPS) treated mouse. Studies were conducted using immunohistochemistry, immunofluorescence, quantitative real time polymerase chain reaction (qRT-PCR) and in vitro tissue cultures. The results showed that the presence of different NOS isoforms in the mouse and human fallopian tubes during different stage of estrous and menstrual cycle; ovarian steroid hormones regulate NOS expression in mouse fallopian tubes. iNOS expression increased in the fallopian tube of human ectopic pregnancies and LPS treated mouse. The results of present study provided morphological and molecular evidences that NOS in the human and mouse fallopian tube might participate in the tubal contractions and suggested that iNOS may be involved in ectopic pregnancies.
Nothing to Disclose: JH