Changes in Blood Glucose, Blood Pressure, Heart Rate and Temperature in Rats Challenged with Either Low Carbohydrate, or High Carbohydrate Treats Following a 12 Hour Fast: Changes Tracked Using Dual Telemetry Implants

Presentation Number: LBSat-47
Date of Presentation: April 2nd, 2016

Scott Tiesma*1, Kimberly L Holliday-White2, Heather M Bogie2, Kathryn E Lillegard2, Ryan Lindquist2 and Bradley W Main1
1Data Sciences International, Saint Paul, MN, 2Data Sciences International, St. Paul, MN

Abstract

Six male Sprague Dawley rats were simultaneously implanted with a telemetry device measuring continuous arterial blood glucose (HD-XG) transmitting at 455KHz, and an second telemetry device measuring arterial blood pressure and a biopotential (ECG) operating at 18MHz (HD-S11-F2).  The HD-XG sensor was placed in the descending abdominal aorta and the device body was located IP. The HD-S11-F2 catheter was inserted in the femoral artery and routed to the abdominal aorta (just proximal to the iliac bifurcation and distal to the glucose sensor. The HD-S11-F2 transmitter body was placed subcutaneously on the flank.   After recovery from surgery, data were collected for several days to observe changes in normal circadian rhythm.  To demonstrate rapid changes in blood glucose while monitoring blood pressure and heart rate, animals were enrolled in a simple cross-over designed study to compare the response to high carbohydrate (marshmallows and fruit) with low carbohydrate treats (cheese cubes) after a 12 hour fast.  Blood pressure, heart rate and blood glucose values are presented in this poster.  Monitoring hemodynamic parameters in addition to blood glucose allowed us to separate excitement responses due to room entry or offering food, and actual changes in blood glucose due to the composition of the food. Room entry had minimal effect on the blood pressure and heart rate following the 12 hour fast, and no observed effect on blood glucose.  Addition of a food offering caused an immediate increase in blood glucose that was related to the carbohydrate load offered by the food.  Animals offered marshmallows and fruit had an immediate increase in blood glucose while those offered cheese had a blunted glucose response.  However, 1 hour after the treats were offered, the normal rat chow was restored to the feeders.  When the normal rat chow was restored, the rats in the marshmallow and fruit group showed little additional increase in blood glucose, while the cheese group had an immediate increase in blood glucose that was even greater than the group that had received the marshmallows and fruit. Blood pressure and heart rate were clearly elevated by adding food to the animals. These experiments demonstrate the value of using two implants to get a more complete picture of whole animal metabolic and cardiovascular physiology.

 

Disclosure: ST: Employee, Data Sciences International (DSI). KLH: Employee, Data Sciences International. HMB: Employee, Data Sciences International. RL: Employee, Data Sciences International. BWM: Employee, Data Sciences International. Nothing to Disclose: KEL