4-Hydroxybenzaldehyde Carries Wound Healing Properties through Activating Focal Adhesion Signaling
Presentation Number: MON 521
Date of Presentation: April 3rd, 2017
Chan Woo Kang*1, Ye Eon Han1, Jean Kim2, Daham Kim3, Yoon Hee Cho4 and Eun Jig Lee5
1Yonsei University College of Medicine and Brain Korea 21 PLUS, Seoul, Korea, Republic of (South), 2Yonsei University College of Medicine and Brain Korea 21 PLUS, 3Yonsei University College of Medicine, Seoul, Korea, Republic of (South), 4Yonsei University, 5Yonsei Univ, Coll of Medicine, Seoul, Korea, Republic of (South)
wound repair is an essential regenerative process, which is required to maintain the barrier function of the skin after damage. This regenerative process is complex with multiple steps that require dynamic interaction of many cell types. Defects in a step of cell migration or angiogenesis often lead to defective wound repair, inducing complications such as diabetic foot ulcer. Despite the importance of the re-epithelialization and neovascularization, our understanding of the molecular control underlying these crucial processes is still lacking. 4-Hydroxybenzaldehyde (4-HBA) is one of the benzaldehydes commonly found in nature. It was originally isolated as one of the main active constituents from Gastrodia elata, which is a very important Chinese herbal medicine. Several reports have mentioned that 4-HBA is active candidate for improving insulin resistance and cholinesterase inhibitor, but there are no reports delineating the therapeutic effects of 4-HBA in skin wound healing. The objective of this study is to determine the effect of 4-HBA on wound healing.
Using in vitroanalysis, we observed that 0.1mM 4-HBA treated HaCaT (immortalized keratinocyte cell line) showed accelerated cell migration and invasion. 0.1mM of 4-HBA had similar keratinocyte migrating effect as much as 0.5mM PDGF-BB, which is the only US PDA-approved growth factor therapy on diabetic wound healing. Interestingly, the combination of 4-HBA and PDGF-BB had additive effect on cell migration. In C57BL7 mice 4-HBA accelerated the wound closure compared to the placebo. Combination of 4-HBA and PDGF-BB resulted in 10% faster closure rate than 4-HBA treated mice and 20% faster than PDGF-BB treated mice. Western blot analysis showed that 4-HBA potently induced phosphorylation of FAK, and SRC, which are the major regulators of migration and invasion.
In conclusion, the current study indicates that 4-HBA may be a possible novel therapeutic reagent for acute wounds and diabetic foot ulcers.
Nothing to Disclose: CWK, YEH, JK, DK, YHC, EJL