No Association Between the Promoter APOC3 Gene rs2854116 and rs2854117 and Risk of Type 2 Diabetes Mellitus, Dyslipidemia, and Non Alcoholic Steatohepatitis (NASH)

Presentation Number: SAT 587
Date of Presentation: April 1st, 2017

Somlak Chuengsamarn*
Division of Endocrinology and Metabolism, Department of Medicine, Faculty of Medicine, HRH Princess Maha Chakri Sirindhorn Medical Center, Srinakharinwirot University,, Nakornnayok, Thailand

Abstract

Apolipoprotein C3 (APOC3) has been relevant to type 2 diabetes (DM) with abnormal lipid profiles and non alcoholic steatohepatitis (NASH). The correlation between APOC3 promoter genotypes and type 2 DM with abnormal lipid profiles and NASH is still controversial, because discrepancies among different studies exist. Ethnic differences play certain roles in these conflicting results, because the distribution of APOC3 promoter polymorphisms is different among study subjects with different racial origins. Therefore, we aim to clarify the association of two SNPs (rs2854116 T>C and rs2854117 C>T) located on the promoter of APOC3 gene in Thai patients with DM together with abnormal lipid profiles and NASH. During 2013-2014, Thai adults aged ≥ 20 years were recruited from Out Patient Department (OPD) of HRN Princess Maha Chakri Sirindhorn Medical Center, and separated into type 2 diabetes (DM) (n=474) and non-diabetes individuals (n=376) in a case-control study. Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP) used for detection of two SNPs in APOC3 gene (rs2854116 T>C and rs2854117 C>T). The result showed that there were no significant associations between these two SNPS and abnormal lipid profiles together with diagnosis NASH by the Liver stiffness (FibroScan) and NAFLD fibrosis score (p>0.05). The APOC3 variant carriers did not increased risk of DM (increased HOMA-IR and higher fasting blood glucose or HbA1c level) (p>0.05). Our study is the first study in Thai population shown the non-relevance between APOC3 gene and DM together with dyslipidemia and NASH. 

 

Nothing to Disclose: SC