Preconception Paternal BPA Exposure Increases Body Weight and Perturbances in Glucose Tolerance in CD1 Mice Offspring

Presentation Number: SAT 249
Date of Presentation: April 1st, 2017

Heather Allardice*1, Alex Mason2 and Emilie F Rissman3
1NCSU, NC, 2NCSU, 3NCSU, Raleigh, NC

Abstract

Bisphenol A (BPA) is a well known endocrine disrupting compound (EDC). In utero exposure to BPA has been implicated in metabolic outcomes such as obesity and glucose perturbances. While the effects of gestational exposure are well established, to date few groups have considered the impact of paternal BPA exposure on F1 offspring. We investigated if preconception paternal BPA exposure affected the body weight and glucose metabolism in the second generation (F1). Twenty-three 60-day-old male CD1 mice (F0) received either a BPA diet (20 μg/day) or vehicle alone for seven weeks. Males were then mated to naïve CD1 females. In order to determine the effects of paternal exposure we made the following pairings: control female X BPA male(CB), control female X control male(CC). The offspring were weighed weekly for eight weeks staring on post natal day seven (PND7). A glucose tolerance test was administered on on PNDs 28, 60, 160. Paternal BPA exposure in the CB group lead to increased body weight for both sexes starting at five weeks (males p=.0134, females p=.00164) indicating a that sex hormones may play an activational role. A decrease in glucose tolerance was observed in males at PND160 (p=.0184) but not in females. Taken together these data provide evidence that paternal exposure to BPA and possibly EDCs in general has the ability to alter metabolic outcomes in subsequent generations and that both age and sex hormones contribute to sexually differentiated metabolic perturbances.

 

Nothing to Disclose: HA, AM, EFR