Glioxal, Methylglyoxal, and 8-Isoprostane As Markers of Oxidative Stress and Lipid Peroxidation in Patients with Diabetes Mellitus and Complications
Presentation Number: MON 506
Date of Presentation: April 3rd, 2017
Armando Gomez-Ojeda*1, Diana Gonzalez-Guerrero1, Claudia Luevano-Contreras2, Carmen Castellanos Pérez-Bolde3, Lizbeth Laredo Mendiola4, Luis Bermúdez Maldonado4, Nayeli Esquitin Garduño4, Katarzyna Wrobel5, Kazimierz Wrobel5 and Ma Eugenia Garay-Sevilla6
1University of Guanajuato, Leon, Gto., Mexico, 2University of Guanajuato, Leon, Gto, Mexico, 3Hospital Regional de Alta Especialidad Bajío, Leon, Gro., Mexico, 4Hospital Regional de Alta Especialidad Bajío, Leon, Gto, Mexico, 5University of Guanajuato, Guanajuato, Gto, Mexico, 6University of Guanajuato, Leon GTO, Mexico
Glyoxal is formed in vivo by lipid peroxidation and degradation of glycated proteins and monosaccharides whilst methylglyoxal is mainly formed by triosephosphate degradation. 8-isoprostane is formed by the oxidation of arachidonic acid mediated by free radicals, and it has been reported as a marker of oxidative stress in the body. These biomarkers are increased in patients with diabetes mellitus (DM), however there are scarce studies about these dicarbonyls in urine. For this reason, the objective of this study was to evaluate glyoxal, methylglyoxal and 8-isoprostane urinary levels in subjects with DM type 2 with or without complications.
Material and Methods: We conducted a cross-sectional study in DM type 2 subjects (n=77) with ≥5 years since diagnosis were classified as having complications (n=51) or without complications (n=21). Glycated Hemoglobin (HbA1c) and lipid profile was measured by standard methods, whilst urinary levels of glyoxal and methylglyoxal were measured by HPLC - FLD, and urinary 8-isoprostane by ELISA. The study was approved by the institutional ethics committee and written informed consent was signed by participants.
Results: When we compare subject with diabetes mellitus without and with complications the subjects with complications had higher levels of HbA1c 6.8 ± 1.4 vs 7.6 ± 1.9 (p<0.018), triglycerides 138.6 ± 56.3 vs 182.2 ± 85.8 (p<0.02), and urinary levels of glyoxal 29.3 ± 20.3 vs 47.9 ± 21.4 (p <0.002); methylglyoxal was also higher, but no significant differences were observed. In contrast, lower urinary levels of 8-isoprostane 1227.6 ± 764.0 vs 1132.1 ± 413.7 (p <0.031) were found in subjects with complications. In the overall group glyoxal correlated with the years since diagnosis p <0.004, triglycerides p <0.038, cholesterol p <0.011 and methylglyoxal p <0.00001.
Conclusions: Urinary levels of glyoxal and methylglyoxal are higher in subjects with DM type 2 with complications, but urinary levels of 8-isoprostane was decreased in comparison with those without complications.
Nothing to Disclose: AG, DG, CL, CC, LL, LB, NE, KW, KW, MEG