Tissue-specific expression of claudins in the various organs of mouse

Presentation Number: SAT-410
Date of Presentation: June 15th, 2013

Inho Hwang and Eui-Bae Jeung*
College of Veterinary Med, Cheongju Chungbuk, Korea, Republic of (South)

Abstract

Claudins (CLDNs) are tetraspan transmembrane proteins that form tight junctions. The CLDN family is composed of 27 members that are responsible for paracellular transport, and some CLDNs form charge-selective ion channels. CLDNs have two extracellular loops, and the charge of the first extracellular loop determines the ion selectivity of each CLDN. Although the expression and function of CLDNs have been progressively studied in single tissues, the tissue distributions of CLDNs are not well established. In this study, the tissue-specific mRNA distributions of 16 CLDNs (1, 2, 3, 4, 5, 7, 8, 10a, 10b, 11, 12, 14, 15, 16, 17, and 19) were investigated by real-time PCR in the duodenum, ileum, colon, kidney, liver, lung, brain, and skeletal muscle. Expression of CLDN mRNA was detected in all tested tissues, except CLDN10a, 16, and 19. CLDN10a and 16 were expressed only in the kidney and liver. CLDN19 mRNA was not detected in the ileum and colon. The translational levels of CLDNs were corresponded well to those of mRNA. CLDN1, 2, 4, 12, 16, and 19 were localized to intercellular apical junctions in all the tested tissue. These findings suggested that the various tissue distributions and expression patterns of CLDN members may indicate distinct rols depending on the tissue.

 

Nothing to Disclose: IH, EBJ